Fluorometric Determination of Pyridoxal and its 5′-Phosphate in Biological Materials
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چکیده
منابع مشابه
Preliminary studies for fluorometric determination of pyridoxal and of its 5'-phosphate.
Bonavita (1) found that the reaction of pyridoxal (PAL) and of pyridoxal 5•Œ-phosphate (PAL-P) with cyanide in a slightly alkaline solution produced highly fluorescent compounds and this reaction has been applied to fluoro metric determination of PAL and PAL-P as "cyanohydrin method" . Recently it was found that PAL and PAL-P were oxidized with oxygen by a catalytic action of cyanide to 4pyrido...
متن کاملBinding of Pyridoxal 5'-Phosphate
1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3. The protein fluorescence of the apo(a subform) decreased non-linearly with increase in enzyme activity and concentration of bound cofactor. 4. It is concluded that the...
متن کاملMOLECULAR MODELING AND NMR STUDY OF HISTDINIE AND ITS ANALOGUES AS , PYRIDOXAL 5 '-PHOSPHATE DEPENDENT HISTIDINE DECARBOXYLASE INHIBITORS
Molecular modeling analysis of charge density and heat of fornation by PM3 method as well as C, H NMR and 2D-NMR measurements of histidine (substrate) and some of its derivatives as histidine decarboxylase inhibitors were performed. It was established that the atom, usually nitrogen, which forms internal aldimine with pyridoxal5 -phosphate (PLP), (coenzyme), has negative and almost equal ...
متن کاملTransfer of pyridoxal 5'-phosphate from albumin-pyridoxal 5'-phosphate complex to apo-aspartate aminotransferase.
Pyridoxal 5•L-phosphate (PLP) is known to combine with bovine serum albumin to form a(1:1) complex which scarcely dissociates, even when subjected to intensive dialysis. When this complex was incubated with apo-aspartate aminotransferase (apoGOT) for an appropriate time and the preincubated mixture then submitted to the usual GOT assay, the appearance of GOT activity was obviously confirmed, in...
متن کاملBiomedical aspects of pyridoxal 5'-phosphate availability.
The biologically active form of vitamin B6, pyridoxal 5'-phosphate (PLP), is a cofactor in over 160 enzyme activities involved in a number of metabolic pathways, including neurotransmitter synthesis and degradation. In humans, PLP is recycled from food and from degraded PLP-dependent enzymes in a salvage pathway requiring the action of pyridoxal kinase, pyridoxine 5'-phosphate oxidase and phosp...
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ژورنال
عنوان ژورنال: THE JOURNAL OF VITAMINOLOGY
سال: 1970
ISSN: 0022-5398
DOI: 10.5925/jnsv1954.16.132